Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.
Applications:
Suitable for use in ELISA and Immunofluorescence. Other applications not tested.
Recommended Dilution:
ELISA: 1:2,000-1:8,000
Immunofluorescence: 1:10-1:40
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, dH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.