Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.
Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry, ELISA and Dot Blot. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Frozen): 1:20 if Peroxidasae labeled secondary is used. If FITC is used dilute 1:10. Not tested on paraffin.
Immunocytochemistry: Cultured bovine cells
ELISA: Use on native bovine collagen I and III.
Dot Blot: Use on native bovine collagen I and III.
Optimal dilutions to be determined by the researcher.
IHC Positive Control:
Bovine kidney, skin
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with 1ml ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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