Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.
Applications:
Suitable for use in Immunofluorescence, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunofluorescence: 1:10-1:40
ELISA: 1:500-1:2,000
Immunohistochemistry (frozen): 1:10-1:40
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.