Collagen XIII A1 (Col13a1, Collagen alpha-1(XIII) Chain)

Collagen XIII A1 is an 85-95kD protein in the type 2 transmembrane collagen family. Mature mouse Collagen XIII A1 consists of a 40aa cytoplasmic domain, a 19aa transmembrane segment, and a 692aa extracellular domain (ECD). The ECD contains three collagenous regions separated by shorter non-collagenous regions. Within comparable regions of the ECD, mouse Collagen XIII A1 shares 85% and 88% aa sequence identity with human and rat Collagen XIII A1, respectively. Mouse Collagen XIII A1 is extensively spliced, with some isoforms showing a tissue specific distribution. Collagen XIII A1 is widely expressed during development and in the adult. It localizes to intercellular adherens junctions and cell-matrix focal adhesions. Collagen XIII A1 assembles into disulfide-linked trimers, a process that is enhanced by proline hydroxylation. Trimerization involves triple helix formation within the collagenous domains, although portions of the non-collagenous regions can also form coiled coils. The ECD of trimeric Collagen XIII A1 is an extended rod-like structure with two flexible hinges that correspond to non-collagenous regions. Collagen XIII A1 clusters in cholesterol-rich domains on the plasma membrane, and it can be cleaved from the cell surface or intracellularly by a furin-like protease. Collagen XIII A1 binds the extracellular matrix molecules fibronectin, heparin, integrin A1 nidogen-2, perlecan. The shed ECD retains its ability to bind fibronectin and can interfere with matrix formation.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nImmunohistochemistry: 5-15ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7510-94B

Size

100ug

Applications

IHC, WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Franzke, C.-W. et al. (2005) J. Biol. Chem. 280:4005. 2. Hagg, P. et al. (1998) J. Biol. Chem. 273:15590. 3. Pihlajaniemi, T. and M. Tamminen, 1990, J. Biol. Chem. 265:16922. 4. Peltonen, S. et al. (1997) DNA Cell Biol. 16:227. 5. Sund, M. et al. (2001) Matrix Biol. 20:215. 6. Peltonen, S. et al. (1999) J. Invest. Dermatol. 113:635. 7. Hagg, P. et al. (2001) Matrix Biol. 19:727. 8. Snellman, A. et al. (2000) J. Biol. Chem. 275:8936. 9. Latvanlehto, A. et al. (2003) J. Biol. Chem. 278:37590. 10. Snellman, A. et al. (2007) J. Biol. Chem. 282:14898. 11. Tu, H. et al. (2002) J. Biol. Chem. 277:23092. 12. Vaisanen, T. et al. (2006) J. Biol. Chem. 281:33352. 13. Nykvist, P. et al. (2000) J. Biol. Chem. 275:8255. 14. Vaisanen, M.-R. et al. (2006) Biochem. J. 393:43.

Additional Info

Recognizes mouse Collagen XIII A1. Species sequence homology: Recombinant human COL13A1v4 (35%), rhCOL4A1, rhCOL25A1, rhCOL3A1, and rhCOL1A1 (1%).