Collagenases degrade native helical collagen fibrils.The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. Suitable for use in cell dissociation procedures.
Collagenase Nomenclature:
Type 1-Containing average amounts of assayed activities (collagenase, caseinase, clostripain and tryptic activities). It is generally recommended for fat, adrenal, and liver cells.
Type 2-Containing greater clostripain activity. It is generally used for heart, bone, muscle, thyroid, cartilage, and liver cells.
Type 3-Containing low proteolytic activity. It is usually used for mammary and fetal cells.
Type 4-Containing low tryptic activity. It is commonly used for islets and other applications where receptor integrity is crucial.
Absorbance:
As reported
Activity:
(same/more than) 500 units/mg dry weight (dw)
(same/less than) 50 units/mg Caseinase (dw)
(same/less than) 2.0 units/mg Clostripain (dw)
(same/less than) 0.25 units/mg Tryptic (dw)
Unit Definition:
1 unit releases 1umole of L-leucine equivalents from collagen in 5 hours at 37C, pH 7.5.
Quality Control:
SDS-PAGE
Storage and Stability:
Collagenase is stable for 6 months at 4C.
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