Formulated to enhance recovery of aerobic and anaerobic organisms from blood cultures.
Eller et al. first described Columbia Broth. It was designed to produce rapid and luxuriant growth of a range of organisms due to its high nutrient content. It contains meat peptone, casein peptone and yeast extract all of which are highly nutritious components. The meat peptone contains sufficient sulfonamide antagonists to prevent sulfonamide inhibition of growth. Columbia Broth, in the presence of CO2 and supplemented with SPS, is an excellent blood culture medium.
Storage:
Store the sealed bottle containing the dehydrated medium at 2 to 30.0 degrees C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.
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