Complement 1s (Complement Component 1 s subcomponent, Basic proline-rich peptide IB-1, C1 esterase, Complement C1s subcomponent precursor)

Activated C1s is a glycosylated single-polypeptide zymogen, MW 85 kD. Activation of the proenzyme C1s occurs through cleavage by the active form of C1r. The activated protease, activated C1s, consists of a disulfide-linked H chain and a L chain. Activated C1s is a serine protease and its catalytic site is located in the L chain. Activation of the classical C pathway is triggered by activated C1s which cleaves C4 and C2 to form the C3 convertase, C4bC2a. The epitope recognized by the antibody M81 is domain IV and/or V of the gamma-domain of activated C1s. Monoclonal antibody M81 blocks C4 activation and C4 binding to activated C1s. The antibody reacts around the binding site of C1s and reacts with both active and inactive C1s.\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry (frozen and paraffin), Immunoassays. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:10\nWestern Blot: 1:10\nImmunohistochemistry: 1:10\nImmunoprecipitation: 1:10\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7850-08F

Size

100ug

Applications

FC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1.Matsumoto, M et al; Probing the C4-binding site on C1s with monoclonal antibodies, evidence for a C4/C4b-binding site on the gamma-domain. J Immunol 1989, 8: 2743 2.Nakagawa, K et al; Complement C1s activation in degenerating articular cartilage of rheumatoid arthritis patients: immunohistochemical studies with an active form specific antibody. Ann Rheum Dis 1999, 58: 175

Additional Info

Recognizes an epitope on human C protein activated C1s, a subcomponent of the first component of C (C1). C1s is capable of inhibiting C4 binding and activation.