Complement 1s (Complement Component 1 s subcomponent, Basic proline-rich peptide IB-1, C1 esterase, Complement C1s subcomponent precursor)

Activated C1s is a glycosylated single-polypeptide zymogen of 85 kD. Activation of the proenzyme C1s occurs through cleavage by the active form of C1r. The activated protease, activated C1s, consists of a disulfide-linked H chain and a L chain. Activated C1s is a serine protease and its catalytic site is located in the L chain. Activation of the classical C pathway is triggered by activated C1s which cleaves C4 and C2 to form the C3 convertase, C4bC2a. The epitope recognized by the antibody M241 is domain IV and/or V of the gamma-domain of activated C1s. Monoclonal antibody M241 blocks C4 activation and C4 binding to activated C1s. The antibody binds specifically to the active centre of C1s.\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry (frozen and paraffin), Immunoassays. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:10\nWestern Blot: 1:10\nImmunohistochemistry: 1:10\nImmunoprecipitation: 1:10\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7850-08G

Size

100ug

Applications

FC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1.Matsumoto, M et al; Probing the C4-binding site on C1s with monoclonal antibodies, evidence for a C4/C4b-binding site on the gamma-domain. J Immunol 1989, 8: 2743 2.Nakagawa, K et al; Complement C1s activation in degenerating articular cartilage of rheumatoid arthritis patients: immunohistochemical studies with an active form specific antibody. Ann Rheum Dis 1999, 58: 175

Additional Info

Recognizes an epitope on human C protein activated C1s, a subcomponent of the first component of C (C1).