Complement C1q, Goat

C1q is useful for the detection of circulating immune complexes. The formation of immune complexes by the interaction of antibodies with antigens is a component of the normal immune response. However, immune complexes occasionally trigger a sequence of events that lead to disease. Circulating immune complexes appear in elevated levels in a variety of diseases (e.g. rheumatoid arthritis, systemic lupus erythematosus, glomerulonephritis, and schistosomiasis). (1-5) \n\nThe first component of the classical complement pathway is C1. C1 is a complex of three proteins, C1q, C1r, and C1s. The complete C1 complex is made up of one C1q, two C1r, and two C1s proteins.\n\nThe classical complement pathway is activated when C1q binds to the Fc regions of IgG or IgM bound to antigen. Although the binding of C1q to a single Fc region is weak, C1q can form tight bonds to a cluster of Fc regions. At this point C1 becomes proteolytically active. C1 can also activate spontaneously at 37?C. Spontaneous activation is inhibited by C1 inhibitor.\n\nA glycoprotein, C1q is approximately 8% carbohydrate with the molecular weight of 460,000. (6) Recent developments in ELISA methodology have increased the sensitivity and therefore the diagnostician's ability to quantitate circulating immune complexes using C1q.

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SPECIFICATIONS

Catalog Number

C7850-06F1

Size

50ul

Applications

ELISA, WB

Form

Supplied as a frozen liquid in 0.05M Tris, 0.5M sodium chloride, 1mM EDTA, pH 7.2.

Purity

C1q is dissociated from the C1 complex and purified by a combination of chromatographic and biochemical processes. This product precipitates heat aggregated human IgG in a double immunodiffusion assay.

References

1. Espinoza, L.R., and Osterland, C.K. (editors). \"Circulating Immune Complexes. Their Clinical Significance.\" Futt, Publishing Co., NY, 1983, pg 1-319. \n2. Peeters, H. (editor). \"Immune Complexes in Protides of the Biological Fluids.\" Vol: 26, Pergamon Press, N. Y. 1979, pg 448. \n3. Theofilopoulos, AN., and Dixon, F.J. \"The Biology and Detection of Immune Complexes.\" Adv. Immuno. Vol: 28, pg 89-220. \n4. Williams, Jr., R. C. \"Immune Complexes in Clinical and Experimental Medicine.\" Harvard University Press, Cambridge, Massachusetts, 1980, pg 1-565. \n5. World Health Organization, \"The Role of Immune Complexes in Diseases.\" , W.H.O. Technical Series 606 , 1977. \n6. Schumaker, Verne N., Podack, Eckhard R. (editor), \"Cytolytic Lymphocytes and Complement: Effectors of the Immune System.\", Vol: 1. CRC Press, 1988, pg 4-16.