Complement C1q, Human

Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. The switch from energy storage to expenditure is finely regulated by a variety of hormones. In order to accomplish these complex tasks energy balance, adipocytes express many genes, including adipsin, involved in lipid metabolism and glucose homeostasis. Many of these genes are finely regulated during adipocyte differentiation and maturation. Several adipocyte-derived proteins act in an autocrine or paracrine fashion to control its own and other cell's cellular physiology.\n\nAcrp30 (adipocyte complement-related protein of 30kD), also known as AdipoQ, APM1, Adiponectin, Gelatin binding protein 28kD/GBP28 or adipocyte most abundant gene transcript) was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, Acrp30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. C1q is serum glycoprotein of 18-polypeptides chains consisting of three non-identical subunits, A (29kD), B (26kD), and C (246 aa, 19kD) in molar ratio of 1:2:2. C1q in the plasma is complexed with two proenzymes C1r and two C1s molecule to form the first component of complement (C1). Activation of complement via classical pathway is triggered by binding of globular head of C1q to immune complexes containing IgG (Fc-region) or IgM or to a variety of other activating substances, including C-reactive protein, retrovirus, and mitochondria. Subsequent to C1q binding, c1r and C1s are converted to proteolytic enzymes that are responsible to continuation of activation via the classical pathway.\n\nApplications: \nSuitable for use in Western Blot as a positive control. Not suitable for ELISA. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 10ul/lane\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7850-06F

Size

50ul

Applications

WB

Reactivities

Hum

Form

Supplied as a liquid in SDS-PAGE sample buffer pH 7.2

Purity

Purified

References

1. Loos et al (1980) J. Immunol. 124, 59; 2. Kolb WP et al (1979) J. Immunol. 122, 2103; 3. Petry F et al (1996) Immunogenetics 43, 370; 5. Scherer PE et al (1995) JBC 270, 26746; 6. Hu E et al (1996) JBC 271, 10697; 6. Das K et al (2001) BBRC 280, 1120; 7. Fruebis J et al (2001) PNAS 98, 2005; Maeda K et al (1996) BBRC 221, 286, 8. Schaffler A et al (1998) BBA 1399, 187; 9. Schaffler A et al (1999) BBRC 260, 416.

Additional Info

A single precipitin arc against the whole human serum proteins in immunoelectrophoresis