The complement component C1 is a protein complex that consists of six subunits of C1q.Each C1q subunit of the complex is known to bind to immune complexes, which often are deposited in the basement membrane.C1q is a versatile recognition protein that triggers activation of the classical pathway of complement, where the first step involves the binding of the globular
domain of Clq to the Fc regions of aggregated IgG or IgM.The C1q globular domain is a heterotrimer of the C-terminal halves of one A, one B, and one C chain, held together
mainly by non-polar interactions, with a Ca2+ ion bound at the top.A modular organization has been suggested of the globular domain, consistent with the view that A, B, and C chains are functionally autonomous modules and have distinct and differential ligand-binding properties (1-3).
Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:500-1:1000
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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