Home  >  Products  >  Complement C3, Activated

Complement C3, Activated

Cat no: C7850-10A1


Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. Activation products of the complement cascade contain neo-epitopes that are not present in the individual native components. Monoclonal antibodies detecting neo-epitopes have been used for direct quantification of activation at different steps in the complement cascade. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3mg/ml. An inherited deficiency of C3 predisposes the person to frequent assaults of bacterial infections. In ulcerative colitis and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported. Proteolysis by certain enzymes results in the cleavage of C3 into C3a and C3b. C3b becomes attached to immune complexes and is further cleaved into iC3b, C3c, C3dg and C3f. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: ELISA: Detects and/or quantifies C3 activation products in human plasma, synovial fluid and cerebrospinal fluid Immunohistochemistry (Paraffin, frozen): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C7850-10A1
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunohistochemistry
Size: 100ug
Form: Supplied as as liquid in PBS, 0.1% BSA, 0.02% sodium azide.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
References: 1.Garred, P et al; Characterization of a monoclonal antibody MoAb bH6 reacting with a neo-epitope of human C3 expressed on C3b, iC3b, and C3c. Scand J Immunol 1988, 27: 319 2.Halstensen T et al; Epithelial deposition of immunoglobulin G1 and activated complement (C3b and terminal complement complex) in ulcerative colitis. Gastroenterology 1990, 98: 1264 3. Garred P et al; Quantification in enzyme-linked immunosorbent assay of a C3 neo-epitope expressed on activated human complement factor C3. Scand J Immunol 1988, 27: 329 4.Lappega?rd, K et al; Effect of complement inhibition and heparin coating on artificial surface
Additional info: Recognizes a neo-epitope expressed on the cleavage fragments of C3b, iC3b and C3c, but not C3dg and C3f. Species Crossreactivity: Human

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*

Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave