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Complement C3, C3c

Complement C3, C3c

Cat no: C7850-14S

Supplier: United States Biological
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C3c is the major fragment resulting from the C3 cleavage by C3 convertase and factor i. It is composed of an intact beta chain bound to two fragments of the alpha chain. Applications: Suitable for use in Immunoelectrophoresis and ELISA. Other applications not tested. Recommended Dilution: Immunoelectrophoresis: 120ul against 2ul canine plasma or equivalent Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7850-14S
Reactivities: Canine
Hosts: Goat
Applications: ELISA
Size: 1ml
Form: Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
P type: Pab
Isotype: IgG
Purity: Serum
Additional info: Recognizes canine C3, C3c. In IEP against fresh canine serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Also reacts with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. Does not react with any other proteins component of canine serum or plasma.

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