Home  >  Products  >  Complement C3, C3d (Complement C3d Fragment, Complement Component C3d, C3/C3d, Complement Component 3, Complement Factor 3, CPAMD1)
Complement C3, C3d (Complement C3d Fragment, Complement Component C3d, C3/C3d, Complement Component 3, Complement Factor 3, CPAMD1)

Complement C3, C3d (Complement C3d Fragment, Complement Component C3d, C3/C3d, Complement Component 3, Complement Factor 3, CPAMD1)

Cat no: C7850-14Y5

Supplier: United States Biological
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C3d is a component of complement. Cell-bound C3b may undergo limited proteolysis to release the fragment C3c and retain C3d on the membrane. Applications: Suitable for use in ELISA, Immunoelectrophoresis and Single and Double Radial Immunodiffusion (Ouchterlony). Other applications not tested. Recommended Dilution: Immunoelectrophoresis: 120ul against 2ul human plasma or equivalent Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul plasma samples (neat and serially diluted) in 2mm diameter peripheral wells. Single Radial Immunodiffusion: use 1% antiserum in the gel Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7850-14Y5
Reactivities: Human
Hosts: Goat
Applications: ELISA
Size: 1ml
Form: Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
P type: Pab
Isotype: IgG
Purity: Serum
Additional info: Recognizes human C3d. In immunoelectrophoresis and double radial immunodiffusion against normal human serum, a single precipitin line is obtained and giving a reaction of full identity with the purified immunogen. Also reacts with fragments bearing common determinants: C3, C3b, C3bi. Does not react with any other proteins component of human serum or plasma. In supernatants of serum containing 10% polyethyleneglycol only a reaction with C3d has been obtained.

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