Complement C3

C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185kD and it consists of alpha and beta chains (110kD and 75kD respectively) held together by disulfide bonds.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:4000\nWestern Blot: 75kD beta-chain band on SDS-PAGE Western Blot of reduced C3 and 20kD and 17kD beta-chain fragments produced by cyanogen bromide cleavage\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nX63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7850-10G

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.\n

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography from culture supernatant.

Isotype

IgG2a,k

References

1. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford.\n2. Morley BJ and Walport MJ (2000) The Complement FactsBook. Academic Press, London, UK.\n3. Koch C, Behrendt N (1986) A novel polymorphism of human complement component C3 detected by means of a monoclonal antibody. Immunogenetics 23:322-325.\n4. Behrendt N, Hansen OC, Ploug M, Barkholt V, Koch C (1987) Localization and functional significance of a polymorphic determinant in the third component of human complement. Mol Immunol 24:1097-1103.\n5. Botto M, Fong KY, So AK, Koch C, Walport MJ (1990) Molecular basis of polymorphisms of human complement component C3. J Exp Med 172:1011-1017.

Additional Info

Recognizes an allotypic marker on human C3 (Leu instead of Pro in codon 314 of exon 9), which occurs regularly in the beta-chain of C3F and occasionally in the beta-chain of C3S.