Complement C3

C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185kD and it consists of two chains (110kD and 75kD) held together by disulfide bonds.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:15,000\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nX63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.\n\nCulture Medium:\nDulbecco

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SPECIFICATIONS

Catalog Number

C7850-10J

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.\n

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography from culture supernatant.

Isotype

IgG1,k

References

1. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford. 2. Morley BJ and Walport MJ (2000) The Complement FactsBook. Academic Press, London, UK. 3. Nagar B, Jones RG, Diefenbach RJ, Isenman DE, Rini JM (1998) X-ray crystal structure of C3d: a C3 fragment and ligand for complement receptor 2. Science 280:1277-1281.

Additional Info

Recognizes human C3d, a fragment of the alpha chain. Epitope specificity differs from that of clones 6F63, 6F64, and 6F65 but overlaps slightly as determined by inhibition ELISA. Reacts strongly with C3. Strong reaction is seen in ELISA with C3 directly coated onto the microtiterwell, or when used as detection antibody in sandwich ELISA in combination with a polyclonal antibody against C3. In Western Blot after SDS-PAGE, this antibody reacts with C3 in reduced as well as nonreduced forms.