Complement C3

C3 is a key component of the complement system since the classical and the alternative activation pathways merge at the C3 activation step. The innate immune system including the complement system was the first to emerge before adaptive immune responses evolved, thus the components involved are very similar in physological different species.\n\nApplications: \nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:2000\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nX63-Ag8.653. Female CF1 x Balb/c mice immunized i.p. with immunogen adsorbed onto Al(OH)3.\n\nCulure medium: RPMI 1640 with 10% fetal calf serum\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7850-10K

Size

100ul

Applications

ELISA, IHC

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide, before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography from culture supernatant.

Isotype

IgG1,k

References

1. Kemp E, Dieperink H, Leth P, Jensenius JC, Nielsen B, Lillevang ST, Salomon S, Steinbruchel D, Larsen S, Koch C, et al. (1994) Monoclonal antibodies to complement C3 prolong survival of discordant xenografts: guinea pig heart to rat transplantation. Transplant Proc 26:1011-1015.\n2. Nielsen B, Steinbruchel DA, Lillevang ST, Salomon S, Kemp E (1994) Natural history of hamster heart rejection when transplanted to rat: a pathoanatomical study including immunohistochemistry. Transplant Proc 26:1024-1025.

Additional Info

Recognizes the complement component of rat C3. No cross-reaction is seen when tested with human C3.\nReacts strongly with rat C3. Strong reaction is seen when tested in sandwich ELISA in combination with a polyclonal antibody against rat C3. Administered to rats transplanted with guinea-pig hearts it prolongs xenograft survival.