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Complement C3, Human

Complement C3, Human

Cat no: C7850-10P

Supplier: United States Biological
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The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectinactivation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes. Applications: Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1-2ug/ml Immunocytochemistry: 1:200 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906: IgY, Chicken Pab Rb x Ch I1906-10: IgY, Chicken (HRP) Rb x Ch I1906-12: IgY, Chicken (FITC) Rb x Ch I1906-14: IgY, Chicken (AP) Rb x Ch Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C7850-10P
Reactivities: Human
Hosts: Chicken/Bird
Applications: ELISA, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.2. No preservative added.
P type: Pab
Isotype: IgY
Purity: Purified by immunoaffinity chromatography.
Alternative names: Complement component 3, ASP, Complement C3 precursor, CPAMD1, Acylation Stimulating Protein Cleavage Product
References: 1. de Bruijn MH & Fey GH Human complement component C3: cDNA coding sequence and derived primary structure. Proc Natl Acad Sci U S A 82:708-12 (1985).
Additional info: Recognizes human Complement C3 at ~187kD.

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