Complement Component 3a receptor (C3a R) is an ~90kD G-protein coupled 7-transmembrane sulfated glycoprotein expressed on myeloid peripheral blood leukocytes, and on activated lymphocytes, endothelial and internal organ epithelial cells. Engagement of C3a R by C3a activates mast cells and neutrophils, triggering degranulation in airways during asthmatic allergen challenges. It assists in Th2-type inflammatory reactions and stimulates leukocyte chemotaxis. Human C3a R extracellular regions share 50% and 42% aa identity with mouse and rat C3a R, respectively.
Applications:
Suitable for use in ELISA and Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 25ug/ml, 10ul labels 1-2.5 x 10e5 cells in 100-200ul
Optimal dilutions to be determined by the researcher.
Hybridoma:
Mouse myeloma cells with B cells obtained from a mouse immunized with human C3a R transfected NS0 cells.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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