Complement C3b beta, Human

The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. An inherited deficiency of C3 predisposes the person to frequent assaults of bacterial infections. Proteolysis by certain enzymes results in the cleavage of C3 into C3a and C3b. C3b becomes attached to immune complexes and is further cleaved into iC3b, C3c, C3dg and C3f. Furthermore, C3b plays a critical role in the amplification loop of the alternative pathway.\n\nApplications:\nSuitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilutions: \nImmunohistochemistry (Frozen): 1:10. 1hr at RT\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7850-14G

Size

50ug

Applications

ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

US Biological application reference: Li, B. et al., (2009) J Biol Chem. 284:35605-35611. 1. Burger R, et al. 1987. Functional analysis and quantitation of the complement C3 derived anaphylatoxin C3a with a monoclonal antibody. Clin. Exp. Immunol. 68:703-711. 2. Sugita K, et al. 1987. Induction of human complement activation without cytolysis by mouse monoclonal antibodies to human leukocyte antigens. Transplantation. 43:570-574.

Additional Info

Recognizes human complement component C3b beta. Allows demonstration of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. Does not react with C3a or C3d. Inhibits hemolytic function of C3.