Complement C4

C4 and C2 represent substrates for activated C1. Both components are present in serum in an\ninert form. C4 is composed of 3 polypeptide chains of three different types, a, b, g. One of the\nchains is cleaved by the action of activated C1, resulting in activation of C4 exposing an binding\nsite for the next component in the sequence, C2. C4 has a molecular weight of 205000 and is\npresent in plasma in an average concentration of 600mg/ml. The activation of C4 yields two\npeptide fragments: C4a (MW 8000) and C4b (MW 198000). This process shows a considerable degree of similarity to the activation of the components C3 and C5.\n\nApplications: \nSuitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7850-15E

Size

1ml

Applications

ELISA

Hosts

Goat

Reactivities

Hum

Form

Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.

P Type

Pab

Purity

Serum

Isotype

IgG

Additional Info

Recognizes human C4. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony) against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C4 protein. Activated C4b may also react with the antiserum. No reaction is obtained with any other plasma protein component or serum.\nDoes not crossreact with any other component of human plasma.