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Complement C4

Complement C4

Cat no: C7850-15F

Supplier: United States Biological
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C4 and C2 represent substrates for activated C1. Both components are present in serum in an inert form. C4 is composed of 3 polypeptide chains of three different types, alpha, beta, gamma. One of the chains is cleaved by the action of activated C1, resulting in activation of C4 exposing an binding site for the next component in the sequence, C2. C4 has a molecular weight of 205.000 and is present in plasma in an average concentration of 600ug/ml. The activation of C4 yields two peptide fragments: C4a (MW 8000) and C4b (MW 198000). This process shows a considerable degree of similarity to the activation of the components C3 and C5. Applications: Suitable for use in ELISA and Immunoelectrophoresis. Other applications not tested. Recommended Dilution: Immunoelectrophoresis: 120ul against 2ul human plasma or equivalent. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7850-15F
Reactivities: Human
Hosts: Rabbit
Applications: ELISA
Size: 1ml
Form: Supplied as a lyophilized powder. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
P type: Pab
Isotype: IgG
Purity: Serum
Additional info: Recognizes human C4. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C4 protein. Activated C4b may also react with the antiserum. No reaction is obtained with any other plasma protein component or serum.

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