The basic form of complement factor 4 (C4) is part of the classical complement activation pathway. C4 is expressed as a single chain precursor. This is post-tranlationally modified to form a disulphide linked trimer of alpha, beta and gamma chains with an intra-chain thioester bond in the alpha chain, prior to secretion.
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.