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Complement C4d (Gg)

Complement C4d (Gg)

Cat no: C7850-18G

Supplier: United States Biological
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Complement factor C4, formerly known as Gg protein, consists of an alpha-, beta-, and gamma-chain. The classical pathway of complement and the mannose binding lectin (MBL) activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. The latter can be cleaved resulting in C4c and C4d, in which step all functional sites are lost. Therefore, the C4d activation fragment of C4 is an excellent marker for classical complement pathway and MBL pathway activation. In a number of diseases such as rheumatoid arthritis (RA), hereditary angioedema (HAE), systemic lupus erythematosus (SLE) and chronic urticaria with hypercomplementemia levels of C4d are significantly elevated in serum or plasma. C4d levels may also be elevated in plasma from patients with a variety of humoral autoimmune diseases in which complement activation is known to occur. Deposition of C4d in peritubular capillaries has been shown to be a sensitive marker for antibody-mediated (humoral) rejection in renal transplant biopsies. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50 Immunohistochemistry (frozen): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C7850-18G
Reactivities: Human, Guinea Pig
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Zwirner, J et al; Deposition of complement activation products on plastic-adsorbed immunoglobulins. J Immunol Methods 1989, 124: 121. 2. Zwirner, J et al; Classical pathway of complement activation in normal and diseased human glomeruli. Kidney Int 1989, 36: 1069. 3. Zwirner, J et al; Classical pathway of complement activation in mammalian kidneys. Immunology 1993, 80: 162. 4. Feucht, H et al; Capillary deposition of C4d complement fragment and early renal graft loss. Kidney Int 1993, 43: 1333.
Additional info: Recognizes an epitope on the alpha 2 chain of human C4d. It recognizes C4, C4b and C4d. Species crossreactivity: guinea pig

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