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Complement C5

Cat no: C7850-23A

Complement C5

C5 is part of the membrane attack complex (MAC) of the complement system. C5 is split into C5a og C5b by the C5 convertases from the classical and the alternative complement pathway and the C5b fragment initiates the assembly of the terminal MAC (C5b-C9) (1). The molecular mass of C5 is 190kD.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nCulture Medium: \nRPMI 1640 with 10% fetal calf serum.\n\nHybridoma: \nX63-Ag8.653. Female CF1 x Balb c mice immunized by intraperitoneal injection.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7850-23A

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG1,k

References

1. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford.

Additional Info

Recognizes human C5. Epitope specificity differs from that of clone 11F6 but slightly overlaps as determined by inhibition ELISA. Reacts strongly with C5. No reaction seen from patients deficient in C5, when tested in sandwich ELISA in combination with a polyclonal antibody against C5. In Western Blot after SDS-PAGE, reacts with C5 in normal human plasma (PEG precipitated C5) in nonreduced form only, no reaction from C5 deficient patients.

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