Complement C5

C5 is part of the membrane attack complex (MAC) of the complement system. C5 is split into C5a og C5b by the C5 convertases from the classical and the alternative complement pathway and the C5b fragment initiates the assembly of the terminal MAC (C5b-C9) (1). The molecular mass of C5 is 190kD.\n\nApplications: \nSuitable for use in ELISA, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:8000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7850-23B

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.\n

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography from culture supernatant.

Isotype

IgG1,k

References

1. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford.

Additional Info

Specific for human C5. No reaction is seen with plasma from patients deficient in C5.\nEpitope specificity differs from that of clone 6F72 but overlaps slightly as determined by inhibition ELISA.\nReacts strongly with C5. Strong reaction is seen with normal human plasma, and no reaction is seen with plasma from patients deficient in C5, when tested in sandwich ELISA in combination with a polyclonal antibody against C5. In Western blotting after SDS-PAGE this antibody reacts with C5 in normal human plasma (PEG precipitated C5) in nonreduced form only. No reaction is seen with plasma from C5 deficient patients.