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Complement C5, Human

Complement C5, Human

Cat no: C7850-24U1

Supplier: United States Biological
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C5 component of human complement is a plasma protein consisting of two polypeptide chains, a, b, bound by disulphide bonds. It has a molecular weight of 190000. The activated protein forms with the complement factors C6-C9 the membrane attack complex. The average concentration in plasma is about 70mg/ml. Applications: Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested. Recommended Dilution: Immunoelectrophoresis: 120ul against 2ul or equivalent Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10ul in a 3mm diameter center well and 2ul serum samples (neat and serially diluted) in 2mm diameter peripheral wells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7850-24U1
Reactivities: Human
Hosts: Goat
Applications: ELISA
Size: 1ml
Form: Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
P type: Pab
Isotype: IgG
Purity: Serum
Additional info: Recognizes human C5. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony) against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C5 protein. No reaction is obtained with any other plasma protein component or serum.

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