Complement C5a des-Arg (Neo-Epitope)

C5a is a single chain glycopeptide composed of 74aa, which is rapidly converted to the less potent derivate C5a des-Arg upon removal of the C-terminal arginine by carboxypeptidase B. C5a acts as a potent anaphylatoxin by binding to its high affinity receptor C5aR (or CD88) on the membrane of target cells inducing smooth muscle contraction, increased vascular permeability, degranulation of mast cells and basophils, and the release of lysosomal enzymes. In addition C5a stimulates the directed migration of neutrophils, eosinophils, basophils and monocytes. C5a is involved in inflammatory reactions seen in gram-negative bacterial sepsis, trauma, ischemic heart disease, post-dialysis syndrome and a variety of autoimmune diseases. Elevation of C5a is associated with increased cardiovascular risk in patients with advanced atherosclerosis. Also, C5a is closely associated with the capillary leak syndrome in leukemic children after bone marrow transplantation. C5a is also a marker in urine for predicting the onset of acute graft rejection after kidney transplantation and has been shown to be present in extracellular deposits in patients suffering from age-related macular degeneration (AMD).\n\nApplications:\nSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilutions:\nWestern Blot: 1:50. A reduced sample treatment and SDS-PAGE was used and detected a band of ~14kD.\nImmunohistochemistry (Frozen): 1:50. Tissue sections were fixed in Histochoice MB and treated with levamisole to quench endogenouse alkaline phosphatases. The primary antibody was omitted for a negative control.\nOptimal dilutions to be determined by the researcher.\n\nPostive Control: Eye tissue of patient with AMD (1)\n\nNegative Control: Eye tissue of patient without AMD (1)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7850-24G

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

Additional Info

Recognizes a neo-epitope on C5a (des-Arg) that is formed upon cleavage of C5 into C5a and C5b during complement activation. Species Crossreactivity: Human