Complement C5a des-Arg (Neo-Epitope)

C5 is involved in the activation of the lytic pathway within the complement system which is an important factor in innate immunity. The complement pathways can be divided into the activation pathways and lytic pathway. The activation pathways lead, via C3, to the cleavage of the fifth complement component C5. C5a induces smooth muscle contraction, increases vascular permeability, causes degranulation of mast cells and basophils, and release of lysosomal enzymes. In addition C5a stimulates the directed migration of neutrophils, eosinophils, basophils and monocytes. Recent studies indicate the modulation of the acute-phase response in liver and an overall immune response by inducing synthesis of cytokines such as TNF-alpha IL-1beta, IL-6 and IL-8.\nApplications:\nSuitable for use in ELISA, Western Blot, Immunohistochemistry and Functional Studies. Other applications not tested. \nRecommended Dilutions:\nWestern Blot: 1:50\nImmunohistochemistry (Paraffin): 1ug/ml. No pretreatment was used on human tissue sections. Human granulocytes were used as a positive control.\nImmunohistochemistry (Frozen): Tissues sections were fixed in Histochoice MB and blocked with DakoCytomation phosphatases. Pretreatment with levamisole solution was used to quench endogenous phosphatases. Drusen eye sections were used as a positive control and sections lacking the antibody were used as the negative.\nFunctional Studies: Functions as a neutralizing antibody blocking the interaction between C5a and its receptor. Functionally tested by competitive binding studies using C5a and Bt2-cAMP differentiated U937 cells. The biological activity can be defined as the ability of C7850-24H to displace C5a from U937 cell surface.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Human granulocytes\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7850-24H

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Hartmann, H et al; J Immunol Meth 1993, 166: 35. 2, Kola, A et al; Immunotech 1996, 2: 115. 3. Stove, S et al. Clin Diag Lab Immunol 1996, 3: 175. 4. Nozaki, M et al; Proc Natl Acad Sci USA 2006, 103: 2328.

Additional Info

Recognizes a neo-epitope on human Complement C5a (des-Arg) that is formed upon cleavage of C5 into C5a and C5b.