Complement C9 (CT)

Human C9 is a soluble glycoprotein of 61kD. It is the last component in the assembly of the membrane attack complex (MAC). When the complement is activated on target membranes, multiple copies of C9 bind to the C5b-8 complex and assemble the barrel-shaped pore which causes cell lysis. The conformation of C9 changes from globular to a tubular form. The binding of C9 to C5b-8 plays a key role in the function of C9. \n \nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, ELISA, and inhibition of C9 binding to C5b-8. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 1:10\nWestern Blot: 1:10\nImmunohistochemistry (Frozen and paraffin): 1:10\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7850-51A

Size

100ug

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Hatanaka, M et al; Analysis of C5b-8 binding sites in the C9 molecule using monoclonal antibodies: participation of two separate epitopes of C9 in C5b-8 binding. Mol Immunol 1992, 29: 911

Additional Info

Recognizes human Complement C9.

Alternative Names

C-term