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Complement Component 3a (C3a) ELISA Kit

Complement Component 3a (C3a) ELISA Kit

Cat no: ABIN1874277

Supplier: Antibodies-online
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Complement Component 3a (C3a) ELISA Kit: The microtiter plate provided in this kit has been pre-coated with an antibody specific to C3a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm
Catalogue number: ABIN1874277
Size: 96 tests
Antigen: Complement Component 3a (C3a)
Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range: 62.5-4000 pg/mL
Sample type: Serum,Plasma,Biological Fluids

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