Complement Component 4 (C4) BioAssay(TM) ELISA Kit (Mouse)

Complement Component 4 (C4) BioAssay(TM) ELISA Kit (Mouse) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of C4 in mouse serum, plasma and other biological fluids.\n\nDetection Range:\n12.35-1000ng/ml\n\nSensitivity:\n4.79ng/ml\n\nIntra-Assay CV:\n<10%\n\nInter-Assay CV:\n<12%\n\nKit Components:\n*024351A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use\n*024351B: Standard, 2x1 vial \n024351C: Standard Diluent, 1x20ml\n*024351D: Detection Reagent A, 1x120ul \n*024351E: Detection Reagent B, 1x120ul \n024351F: Assay Diluent A, 1x12ml\n024351G: Assay Diluent B, 1x12ml\n024351H: TMB Substrate, 1x9ml\n*024351J: Positive Control 350.8ng/ml, 1x1vial\n024351K: Stop Solution, 1x6ml\n024351L: Wash Buffer, 30X, 1x20ml\n\nPrecaution:\nThe Stop Solution (023393K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.\n\nStorage and Stability:\nStore *024351A, *024351B, *024351D, *024351E and *024351J at -20 degrees C. After reconstitution of *024351J, store at 4 degrees C and use within 5 days. Store all the other components at 4 degrees C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.\n\nNotes on 024351J: Positive Control:\nIntended Use:\nThe control vials are from a mouse serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.\n\nCharacteristics:\nThe control vials contain mouse C4 in mouse serum (lyophilized powder). Note: The mouse based serum used in this product is from a healthy specimen(s) of SPF standard (specific pathogen-free animals).\n\nExpected Value:\n350.8ng/ml\n\nUsage: \nDissolve with 150ul standard diluent and let stand for 10 minutes. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20 degrees C. After reconstitution, store Positive Control vial at 4 degrees C and use within 5 days.\n\nAssay Procedure Summary:\n1. Prepare all reagents, samples and standards.\n2. Add 50ul standard or sample to each well. And then add 50ul of the prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37 degrees C.\n3. Aspirate and wash 3 times.\n4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degrees C.\n6. Aspirate and wash 5 times.\n7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37 degrees C.\n8. Add 50ul Stop Solution. Read at 450nm immediately.

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