Applications:
Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry (frozen and paraffin), and Western Blot. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (frozen): 1:100-1:750
ELISA: 1:5,000-1:20,000
Immunofluorescence: 1:250-1:600
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.