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Complement Factor B, Bb Fragment

Complement Factor B, Bb Fragment

Cat no: C7850-60C

Supplier: United States Biological
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Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry (frozen), and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Kidney from post streptococcal glomerulonephritis patients Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7850-60C
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in borate buffered saline containing 0.09% sodium azide
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography
References: 1. Xu, Y. et al. (2001) Structural biology of the alternative pathway convertase. Immunol. Rev. 180:123-135, 2. Ponnuraj, K. et al. (2004) Structural analysis of engineered Bb fragment of complement factor B: insights into the activation mechanism of the alternative pathway C3-convertase. Mol. Cell. 14:17-28.
Additional info: Recognizes the Bb fragment of human complement factor B. This antibody binds to both the Bb fragment and complement factor B so is not suitable for all ELISA applications.

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