Properdin factor B is a heat-labile serum protein active in the alternative complement
activation pathway. It is binding to the C3b component of complement. The protein has a molecular weight of 92000 and its mean concentration in human plasma is 200ug/ml.
Applications:
Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested.
Recommended Dilution:
Immunoelectrophoresis: 120ul against 2ul human serum.
Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in a 3mm diameter center well and 10ul in 3mm diameter peripheral wells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.