Complement Factor D (also Adipsin, C3 convertase activator and Properdin factor D) is a 37-43kD member of the S1 peptidase family of enzymes. In mouse, it is secreted by fat cells and helps form C3 convertase, a complex of C3b, Factor B and Factor D. This complex cleaves C3 into C3b and C3a, the latter of which is converted into ASP, a molecule that binds the adipocyte receptor C5L2 and induces triglyceride synthesis. Mature mouse Factor D is 234 amino acids in length (aa26-259). In contains one peptidase S1 domain that cleaves an Arg-Lys bond (aa26-254). There are at least two potential alternative splice forms. One shows a two aa substitution for aa 1-71, and a second shows a 15 aa substitution for aa1-107. Mature mouse factor D shares 85% and 68% aa identity with rat and human Factor D, respectively.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:
Western Blot: 0.1ug/ml using recombinant mouse Complement Factor D.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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