Complement Factor H-related protein 1 is a 43kD, secreted member of the factor H family of glycoproteins. It is produced by hepatocytes and circulates as two differentially glycosylated isoforms (37kD and 43kD). Mature human FHR1 is 312aa in length. It contains five, ~60aa SCRs (short consensus repeats/CCPs/SUSHI repeats) that basically constitute the entire molecule. FHR1 may play a role in lipoprotein complexes that bind PMNs to LPS. There is no reported rodent counterpart to FHR1. Over aa 19-143 of the FHR1 precursor, human FHR2 and FHR5 show 98% and 86% aa identity, respectively.
Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions:
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.1ng/well.
Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively.
Immunoprecipitation: Used to immunoprecipitate recombinant human FHR1 from conditioned media of transfected NSO cells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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