Complement Factor I

Factor I, a serine protease, is a regulatory component of both the alternative and the classical complement pathway. Factor I is synthesized as a single chain precursor of 88kD, processed to yield the active form, composed of two disulfide-linked chains, the a-chain (50kD) and the b-chain (38kD) (2). Plasma concentration is 35ug/ml (1).\n\nApplications: \nSuitable for use in ELISA, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7850-90

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography from culture supernatant.

Isotype

IgG1,l

References

1. Morley BJ and Walport MJ (2000) The Complement FactsBook. Academic Press, London, UK.\n2. Law SKA, Reid KBM (1988) Complement. In: In Focus (Ed. Male D) IRL Press: Oxford.\n3. Rasmussen JM (1991) Inherited deficiencies of complement proteins with special reference to congenital deficiency of the regulatory protein factor I. APMIS Suppl 22:1-48.

Additional Info

Specific for the a-chain of human factor I.\nReacts strongly with factor I. Strong reaction is seen with normal human plasma when used in sandwich ELISA in combination with a polyclonal antibody against factor I as capture antbody (e.g. sheep anti human factor I from Serotec). In Western blotting after SDS-PAGE, this antibody reacts with factor I in non-reduced form.