The complement system consists of a number of small proteins found in the blood, generally synthesized by the liver, and normally circulating as inactive precursors (pro-proteins). When stimulated by one of several triggers, proteases in the system cleave specific proteins to release cytokines and initiate an amplifying cascade of further cleavages. The end result of this activation cascade is massive amplification of the response and activation of the cell-killing membrane attack complex. Over 25 proteins and protein fragments make up the complement system, including serum proteins, serosal proteins, and cell membrane receptors. They account for about 5% of the globulin fraction of blood serum.
Mouse Complement is ideal for hemolytic assay where hemolysis of IgG are required. Mouse Complement serum is collected off the clot from healthy fasted mice. Special processing techniques are used to yield products with high complement activity and low background cytotoxicity.
Sex: Mixed
Strain: Mixed
Sterility: Non-sterile
Storage and Handling:
Store at -70 degrees C prior to opening. Aliquot contents and freeze at -70 degrees C or below. Use aseptic technique to maintain sterility when opening product. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
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