Connexin 26 (CX26, Gap Junction Beta 2 Protein, CXB-2)

Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1000D. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to MW, Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane (TM), 2 extracellular (EC), and 3 cytoplasmic (CP) domains. The EC, TM, and N-terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size. The N and C-termini are predicted to be cytoplasmic.\n\nApplications: \nSuitable for use ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using ECL Recognizes a band of ~25kD in mouse liver.\n\nELISA: 1:100,000 using 50-100ng CX26 control peptide/well.\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7853-01A

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Kumar, Nm and Giula, NB (1996) Cell 84, 381-388; Evans, HW (1994) Biocem. Soc. Tr. 788-792; Byer, E et al (1990) J. membrane Biol. 116, 187-194\n2. Henneman, H et al (1992) Eur. J. Cell Biol. 58, 81-89; Nishi, M et al (1991) Dev. Biol. 146, 117-130\n3. Zhang, J-T and Nicholson BJ (1994) J Membrane Biol. 139, 15-29; \n4. Goliger J & Paul D (1994) Devel. Dynamics 200, 1-13\n5. Zhang JT and Nicholson BJ (1989) J cell Biol. 109, 3391-3401; Nicholson B et al (1987) nature 329, 732-734; Lee Sw et al (1992) J Cell Biol. 118, 1213

Additional Info

Recognizes mouse connexin 26. Species sequence homology: rat (100%), monkey (94%), human (89%), sheep, bovine and dog (8%), rat CX30 (63%). No significant hmology with other connexins. \n\n\n