Connexin 26 (CX26, Gap Junction Beta 2 Protein, CXB-2)

Intercellular communication through gap junctions plays an important role in a variety of cellular processes including homeostasis, morphogenesis, cell differentiation, and growth control. (1-4) Gap junctions are transmembrane channels that serve to directly link neighboring cells by mediating the exchange of low-molecular weight (<1200 D) metabolites, ions, and second messengers. Gap junctions are formed by the interaction of hemichannels (connexons) on adjacent cells. The connexon itself is composed of a hexameric assembly of connexin proteins. \n\nApplications: \nSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:500 -1:5000 \nImmuno?uorescence: 1:60-1:250 \nWestern Blot: 1:250-1:1000 Reactivity was confirmed using lysates derived from mouse liver, rat liver and rat brain. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C7853-05

Size

100ug

Applications

ELISA, IF, WB

Hosts

Mouse

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a,k

References

1. Kumar, M. and Gilula, M.B., Cell 84:381:388 (1996). 2. Saez, J.C., et al; In Advances in Second Messenger and Phosphoprotein Research; eds S., Shenolikar and A., Narin. Raven Press, New York (1993). 3. Bennet, M.V.L., et al; Neuron 6:305-320 (1990). 4. Kuraoka, A., et al; J. Histochem. and Cytochem. 41:971-980 (1993). 5. Wilgenbus, KK., et al; Int. J. Cancer 51:522-529 (1992). 6. Dahl, E. et al., J. Biol. Chem. 271:17903-17910 (1996). 7. Nagy, J.I., et al., Neuroscience 78:533-548 (1997).

Additional Info

Recognizes mouse Connexin 26. Does not crossreact with Connexin 30. Species Crossreactivity: rat. Species sequence homology: human.