Connexin 26 (CX26, Gap Junction Beta 2 Protein, CXB-2)

Intracellular communication mediated by gap junctions plays an important role in a variety of cellular processes including: homeostasis, morphogenesis, cell differentiation, and growth control. Gap junctions are transmembrane channels that serve to directly link neighboring cells by mediating the exchange of low-molecular weight metabolites, ions, and second messengers. Gap junctions are formed by the interaction of connexons or hemichannels on adjacent cells. The connexon itself is composed of a hexameric assembly of proteins referred to as connexins. Connexins are highly homologous proteins encoded by a multigene family. The connexins exhibit similar structural features which include a cytoplasmic amino terminal region, four transmembrane domains, two extracellular loops, and a carboxyl-terminal cytoplasmic tail of varying length. Comparison of the aa sequences of the various connexin family members indicate that the two areas of greatest divergence amongst the connexin family members are the intracellular loop connecting the second and third transmembrane segments and the carboxy-terminal tail. These domains are, therefore, thought to mediate connexin-type speci?c properties including: phosphorylation, responses to gating stimuli, as well as assembly and membrane turnover. Modulation of gap junctional communication can be achieved by multiple mechanisms and can occur very rapidly or over a period of several hours. These mechanisms include alterations in transcription, translation, stability, postranslational processing (especially phosphorylation), gating, and insertion or removal from the plasma membrane. Interestingly, reduction or alterations in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis. Mouse connexin 26 is a 226aa gap junction protein with a predicted molecular weight of ~26kD. It is prominently expressed in liver.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 \nELISA: 1:100,000 in 50-100ng C7853-15 Connexin 26, Mouse, Control Peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7853-05A

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Kumar, Nm and Giula, NB (1996) Cell 84, 381-388; Evans, HW (1994) Biocem. Soc. Tr. 788-792; Byer, E et al (1990) J. membrane Biol. 116, 187-194 2. Henneman, H et al (1992) Eur. J. Cell Biol. 58, 81-89; Nishi, M et al (1991) Dev. Biol. 146, 117-130. 3. Zhang, J-T and Nicholson BJ (1994) J Membrane Biol. 139, 15-29. 4. Goliger J & Paul D (1994) Devel. Dynamics 200, 1-13. 5. Zhang JT and Nicholson BJ (1989) J cell Biol. 109, 3391-3401; Nicholson B et al (1987) nature 329, 732-734; Lee Sw et al (1992) J Cell Biol. 118, 1213.

Additional Info

Recognizes mouse Connexin 26. Species sequence homology: rat-100%, human- 90%, sheep-85%.