Connexin 29

Gap junctions have important functions in the transmission of signals across synapses in the nervous system (3). Connexin29 is a gap junction protein of 258 amino acids encoded by a novel single copy mouse gene that shows 75% sequence identity to human connexin 30.2 (4). Connexin29 mRNA (4.4 kb) is highly abundant in mouse sciatic nerve and is less abundant in spinal cord and adult brain (4). Expression data suggest that the new connexin gene is active in myelin-forming glial cells (4).\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 0.5-2ug/ml\nWestern Blot: 0.5-2ug/ml \nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nMouse brain or sciatic nerve homogenates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7853-50

Size

50ug

Applications

ELISA, IF, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4 0.01% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Balice-Gordon R J. et al. J. Cell Biol. 142:1095-1104 (1998). 2. Nagy et al., under preparation. 3. Rozental et al. Brain Res Brain Res Rev. 32: 11-15 (2000). 4. Sohl G et al. Biol. Chem. 382: 973-978 (2001).

Additional Info

Recognizes mouse Cx29. Peptide inhibition studies were used to con?rm the antibody