Connexin 32 (Cx32)

Intercellular communication mediated by gap junctions plays an important role in a variety of cellular processes, including homeostasis, morphogenesis, cell differentiation, and regulation of growth.1пїЅ3 Gap junctions are transmembrane channels that directly link neighboring cells by regulating the exchange of low molecular weight (<1200D) metabolites, ions, and second messengers. Gap junctions are formed by the interaction of hemichannels (connexons) on adjacent cells. Each connexon is composed of a hexameric assembly of connexin proteins. Connexins are highly homologous proteins encoded by a multigene family with similar structural features, including a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops, and a C-terminal cytoplasmic tail. Comparison of the amino acid sequence of the various connexin family members indicates that the two areas of greatest divergence are the intracellular loop connecting the second and third transmembrane domains and the C-terminal tail.1пїЅ2 These distinct domains are thought to mediate connexin type-specific properties, including phosphorylation, responses to gating stimuli, and assembly and membrane turnover. \n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-5ug/mL\nImmunohistochemistry (4% paraformaldehyde): 0.5-2.0ug/ml. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7854-04

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Kumar M, Gilula MB. Cell 84(3):381-388, 1996. 2. Saez JC, et al. In: Advances in Second Messenger and Phosphoprotein Research. Eds: Shenolikar S, Narin A. New York: Raven Press;163-197, 1993. 3. Kuraoka A, et al. J Histochem Cytochem 41:971-980, 1993. 4. Wilgenbus KK, et al. Int J Cancer 51(4):522-529, 1992. 5. Li J, et al. J Comp Neurol 379(4):571-591, 1997. 6. Rash JE, et al. J Neurosci 21(6):1983-2000, 2001.

Additional Info

Recognizes mouse connexin 32 in liver and brain. On Western Blot with mouse liver cell lysates, Cx32 exhibits a band at 32kD. Reactivity is con?rmed with mouse liver lysates. Species sequence homology: human and rat Cx32.