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Connexin 37(CX37, Gap Junction Alpha 4 Protein, CXA-4)

Connexin 37(CX37, Gap Junction Alpha 4 Protein, CXA-4)

Cat no: C7855-35A

Supplier: United States Biological
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One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. SUBUNIT: A connexon is composed of a hexamer of connexins. Cellular Localization: Membrane; multi-pass membrane protein. Tissue Specificity: Highly expressed in lung. Belongs to the connexin family. Alpha-type (group II) subfamily. Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer..
Catalogue number: C7855-35A
Reactivities: Mouse, Rat
Hosts: Sheep
Applications: Immunofluorescence, Western Blot
Size: 100ul
Form: Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
P type: Pab
Purity: Serum
Alternative names: Gap junction alpha-4 protein, Cx37, Gja4, Cxn-37, CXA-4
References: Rummery et al. (2002). ATVB 22, 1429-1432
Additional info: Western blotting of cell membarnes of COS cells transiently transfected with cDNA encoding Connexin-37 shows two bands of molecular weight 36 and 37kD which are abolished by preincubation with the immunising peptide. No such bands are detected in western blots of membranes from non-transfected cells or cells transfected with cDNA encoding Connexin-40, Connexin-43 or Connexin-45. Species Crossreactivity: This antiserum recognizes rat and mouse Connexin-37 protein.

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