Connexin 37 (CX37, Gap Junction Alpha 4 Protein, CXA-4)

Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1000D. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to MW. Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane (TM), 2 extracellular (EC) and 3 cytoplasmic (CP) domains. The EC, TM and N-terminal CP domains are well conserved among family members. While Central and C-terminal domains are highly variable in both sequence and size. The N- and C-termini are predicted to be cytoplasmic.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 (ECL).\nELISA: 1:10,000-1:100,000. Coat with 50-100ng control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nC7855-37: Connexin 37, Rat, Control Peptide (CX37, Gap Junction Alpha 4 Protein, CXA-4)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C7855-36

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.1% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Kumar, Nm and Giula, NB (1996) Cell 84, 381-388. 2. White , WT et al (1995) Kidney Intl. 48, 1148-1157. 3. Evans, HW (1994) Biochem. Soc. Tr. 788-792. 4. Byer, E et al (1990) J. membrane Biol. 116, 187-194. 4. Wilecke K et al (1991) 114, 1049-1057. 5. Haefliger JA et al (1992) JBC 267, 2057-2064. 6. Reed KE et al (1993) J Clin. Invest. 91, 997-1004.

Additional Info

Recognizes Connexin 37. No significant homology is seen with other Connexin proteins. Species Sequence Homology: Rat Cx37: 93%; human Cx37: 87%; xenopus Cx41: 68%.