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Connexin 37 (CX37, Gap Junction Alpha 4 Protein, CXA-4)

Cat no: C7855-38

Connexin 37 (CX37, Gap Junction Alpha 4 Protein, CXA-4)

Rat Connexin 37 is A 333 aa gap junction protein with a predicted MW. Of ~37kD. It is prominently expressed in lung (see reviews in refs. 1). A 19 amino acid peptide sequence within the C-terminal cytoplasmic domain of rat Cx37 (2) was used to generate anti-Mouse Cx37. Peptide was coupled to KLH (carrier protein) Via the N-terminal Cysteine and antibodies generated in rabbits.\n\nApplications: \nSuitable for use in ELISA, Western Blotting. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: We recommend testing of antiserum at 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using Chemiluminescence technique.\n\nELISA (1:10K-100K using 50-100ng control peptide/well.\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7855-38

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Kumar, N.M. (1996) Cell 84, 381; 2. White ,W.T. (1995) Kidney Intl. 48, 1148; 3. Evans, H.W. (1994) Biochem. Soc. Tr. 788; 4. Byer, E. (1990) J. Membrane Biol. 116, 187-194.

Additional Info

Recognizes rat Cx37. No significant homology is seen with other Connexins. Species Sequence Homology: mouse, 94%; human, 52%.

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