Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1)

Mouse connexin 43 is a 382 amino acid gap junction protein with a predicted MW ~43kD. It is prominently expressed in heart. Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1000 Daltons. The junctions exist in almost all vertebrate and non-vertebrate cells. It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to mol. wt, Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane, 2 extracellular (EC), and 3 cytoplasmic (CP) domains. The EC, TM, and N-terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size. The N and C-termini are predicted to be cytoplasmic.\n\nApplications:\nSuitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and ELISA. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000 using 10ug of rat brain lysates\nImmunocytochemistry: 1:50-1:250\nImmunohistochemistry (Frozen): 1:50-1:250 using mouse keratinocytes and rat heart \nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: C7857-04\nPositive Control: Mouse brain tissue lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7857

Size

100ug

Applications

ELISA, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can, Prc

Form

Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Tansey, Erin E, et al. (2006). Ann. Thorac. Surg. 82: 1472-1479. 2. Arita, Ken, et al. (2006). Am. J. Pathol. 169: 416-423.

Additional Info

Recognizes mouse Connexin 43, Mr 43-47kD. Species Crossreactivity: porcine, rat, human and canine. Species Sequence Homology: bovine. No known homology with other connexins.