Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1)

Gap junctions are transmembrane channels that serve to directly link neighboring cells by mediating the exchange of low-molecular weight metabolites, ions, and second messengers. Gap junctions are formed by the interaction of connexons or hemichannels on adjacent cells. The connexon itself is composed of a hexameric assembly of proteins referred to as connexins. Connexins are highly homologous proteins encoded by a multigene family which exhibit similar structural features including a cytoplasmic amino terminal region, four transmembrane domains, two extracellular loops, and a carboxy-terminal cytoplasmic tail of varying length. The two areas of greatest divergence amongst the connexin family members are the intracellular loop connecting the second and third transmembrane segments and the carboxy-terminal tail. These domains are thought to mediate connexin-type specific properties including phosphorylation, responses to gating stimuli, as well as assembly and membrane turnover. Modulation of gap junction communication can be achieved by multiple mechanisms and can occur very rapidly or over a period of several hours. These mechanisms include alterations in transcription, translation, stability, posttranslational processing (especially phosphorylation), gating, and insertion or removal from the plasma membrane. Interestingly, reduction or alterations in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis.\n\nApplications: \nSuitable for use in ELISA, Western Blot, and Immunoflourescence. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml \nWestern Blot: 1-3ug/ml (Rat)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7857-08

Size

100ug

Applications

ELISA, IF, WB

Hosts

Mouse

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Goodenough DA, et al. Annu Rev Biochem 65: 475-502 (1996). 2.

Additional Info

Reacts with both phosphorylated and non-phosphorylated forms of Connexin 43. Does not detect the Connexin 43 dimer. Detects non-phosphorylated forms migrating at ~41kD and phosphorylated forms migrating between 43kD and 45kD. Species Crossreactivity: Rat and mouse.