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Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1, GJA1, GJAL, Gap junction 43kD heart protein)

Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1, GJA1, GJAL, Gap junction 43kD heart protein)

Cat no: C7857-01M

Supplier: United States Biological
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A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexins, through which materials of low molecular weight diffuse from one cell to a neighboring cell. Each connexin is composed of a hexamer of connexin proteins. Connexins are a multi-gene family of highly related proteins. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages, class I (beta) and class II (alpha), have been identified in mammals. Connexin-43, also known as Gap Junction Alpha-1 (GJA1), belongs to the class II (alpha) group of connexins and is expressed in the heart and fetal cochlea. Applications: Suitable for use in Immunohistochemistry, Western blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml Immunohistochemistry (formalin fixed and paraffin): 1-2ug/ml. Antigen retrieval is required. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Dilute in PBS (pH 7.4). Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C7857-01M
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder in PBS pH 7.4, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute in 100ul of sterile ddH2O.
P type: Pab
Isotype: IgG
Purity: Purified by Immunoaffinity chromatography.
Additional info: Recognizes Connexin-43. Species crossreactivity: human, mouse, rat.

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